Flow cytometric evaluation of CD4+ and CD8+ T-cell in IPB-D2 chickens with different Newcastle disease antibody titers level

Background and Aim: IPB-D2 chickens are selected from IPB-D1 due to their disease-resistance characteristics. One-way evaluate the strength of a chicken’s immune system is by examining number circulating T lymphocytes. This assessment can be conducted using modern analytic method called flow cytometry which relies on monoclonal antibodies detect relative proportions each cell measure quality quantity biological physical features cells, including specific membrane or intracellular glycoprotein markers. Therefore, this study aimed population lymphocytes, cluster differentiation (CD)4+ CD8+ in chickens. Materials Methods: Flow was used CD4+, The data obtained were analyzed Minitab, mean values compared t-test. Results: populations chicken with high Newcastle disease (ND) antibody titers 65.04%, 10.53%, 5.47%. Meanwhile, breed, low ND had lymphocytes CD4+ 57.19%, 8.40%, 4.11 %. comparison 1.92 2.04, respectively. Conclusion: exhibited increased lymphocyte, those titers. However, titer group lower CD4+/CD8+ ratio. Keywords: 4+, 8+, cytometry, chicken,